3D Tumor Cell Models for TME Research

Relying on the tumor microenvironment center technology platform, Alfa Cytology provides custom services for 3D tumor cell models for TME studies, helping researchers successfully achieve their research goals.

Introduction

Tumors involve cellular alterations, including growth and division that take cells out of their normal limits, leading to invasion and destruction of adjacent tissues and eventual metastasis to distant sites in the body. Oncology researchers need better research tools that allow them to more easily study the complex, unknown interactions between tumor cells and their environment and identify points of therapeutic intervention for tumors.

3D cell culture provides cells with a microenvironment that more closely resembles in vivo survival conditions and can better mimic physiological states, establishing physiological cell-cell and cell-extracellular matrix interactions that mimic the specificity of natural tissues. Moreover, in 3D cell culture, cell adsorption can occur on the entire cell surface, and the degree of cell adsorption and extension affects its proliferation, apoptosis and differentiation functions. All of these can lead to more consistent experimental results with in vivo experiments for 3D cultured cells. With improvements in biorelevance, ease of use and throughput of several new 3D cell culture techniques, this technology is increasingly being used in basic research and drug discovery.

Services

At Alfa Cytology, we have established a mature tumor microenvironment center technology platform with a group of experienced tumor model customization experts and proven technologies designed to provide our global customers with efficient and validated tumor models for tumor and microenvironment research and antitumor drug discovery.

Alfa Cytology offers 3D cell model construction services, covering cell lines, substrates and consumables, media systems, and platforms and reagents for imaging analysis to help you explore the relationship between tumor development and the microenvironment.

The main types of 3D cultured cells that we recommend for tumor and microenvironment research are:

• Pluripotent stem cells
• Skin cells
• Cancer spheroids
• Liver cells
• Brain cells

Service content / Model construction steps

1. Cell source. Select a cell line that closely mimics the in vivo state and generates more relevant data.

2. Scaffolding/support. Choosing the right culture matrix is an important start to successfully developing a 3D culture system.

3. Cell culture. The 3D cell culture system can be modified and developed to accommodate specialized cell types, such as stem cells or cancer cell lines.

4. Monitoring and analysis. Visualization and quantitative analysis of 3D cell models.

5. Characterization and analysis. Molecular characterization of 3D spheroid models and applications.

Note: Our services list is constantly being updated and improved, please contact us by email for more up-to-date and relevant information.

Service process

With extensive experience in various cancer models and expertise in tumor and microenvironment, Alfa Cytology is the ideal partner to support your oncology therapeutic research. We provide customers with stock model products, model customization, and joint R&D services to provide you with the best tumor models and look forward to meeting your precise needs. Please let us know your project requirements and we will provide you with a comprehensive service from solution to report. If you have any questions, please feel free to contact us.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.